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  • Title: Transient increase of cytosolic free calcium in cultured human vascular endothelial cells by platelet-activating factor.
    Author: Hirafuji M, Maeyama K, Watanabe T, Ogura Y.
    Journal: Biochem Biophys Res Commun; 1988 Aug 15; 154(3):910-7. PubMed ID: 3408504.
    Abstract:
    The effect of platelet-activating factor (PAF-acether) on cytosolic free calcium, [Ca2+]i, in adherent human vascular endothelial cells in culture was directly determined using a new fluorescent calcium indicator, fura-2. It was found that PAF-acether but not lyso PAF-acether induced a rapid and transient increase in [Ca2+]i in endothelial cells. Restimulation with PAF-acether after the first challenge did not cause further response, while the cells were able to respond to thrombin. In the absence of extracellular calcium, PAF-acether evoked a similar transient increase, suggesting that PAF-acether raises [Ca2+]i mainly by discharging calcium from intracellular pools. PAF-acether-induced rise in [Ca2+]i was completely blocked by a specific antagonist, BN 52021. These results suggest the receptor-mediated increase in [Ca2+]i as an early event in PAF-acether activation of human vascular endothelial cells.
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