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  • Title: Label-free photoelectrochemical immunosensor for aflatoxin B1 detection based on the Z-scheme heterojunction of g-C3N4/Au/WO3.
    Author: Pei F, Feng S, Wu Y, Lv X, Wang H, Chen SM, Hao Q, Cao Y, Lei W, Tong Z.
    Journal: Biosens Bioelectron; 2021 Oct 01; 189():113373. PubMed ID: 34090152.
    Abstract:
    Aflatoxin B1 (AFB1) is the most toxic mycotoxin, is widely found in foods and animal feeds, and can pose a serious threat to our lives. A label-free photoelectrochemical (PEC) immunosensor was fabricated for the sensitive detection of AFB1. A Z-scheme heterojunction of gold nanoparticles (Au NPs) loaded on graphitic carbon nitride sheet and tungsten trioxide sphere composite (g-C3N4/Au/WO3) acted as the highly sensitive platform. The g-C3N4/Au/WO3 is capable, not only of immobilizing antibodies via Au NPs, but also enhancing the separation of electron-hole pairs due to its good energy band matching efficiency. The mechanism of photo-generated electron/hole transfer on g-C3N4/Au/WO3 was explored using scavengers to eliminate active components. On this basis, an electron transfer pathway for the immunosensor was deduced. The PEC immunosensor displayed a linear concentration range from 1.0 pg mL-1 to 100 ng mL-1 and a low detection limit of 0.33 pg mL-1 (S/N = 3) for AFB1. Good reproducibility, stability, and specificity provide a solid foundation for the practical application of this immunosensor.
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