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  • Title: Isoelectric focusing in immobilized pH gradients of a snake venom fibrinolytic enzyme.
    Author: Guan AL, Markland FS.
    Journal: J Biochem Biophys Methods; 1988; 16(2-3):215-26. PubMed ID: 3411084.
    Abstract:
    A fibrinolytic enzyme with a molecular weight between 23,000 and 25,000 Da has been purified from southern copperhead snake venom. Immobilized pH gradient isoelectric focusing with an ultranarrow pH interval (pH 6.65-6.95) resolved two isoforms of the fibrinolytic enzyme that were not resolved by standard isoelectric focusing. Attempts at purification of the individual isoenzymes by semi-preparative scale IPG and elution of enzyme by macerating the gel yielded only 20-40% recovery of activity. In attempts to improve recovery, a semi-preparative IPG canal-isoelectric focusing technique has been utilized.
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