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  • Title: [Effect of moderately low temperatures (-30 C to -70 C) on the permeability of erythrocyte membranes].
    Author: Kukushkin AI, Gulevskiĭ AK, Loevskiĭ MM, Makarenko VV.
    Journal: Ukr Biokhim Zh (1978); 1988; 60(3):104-6. PubMed ID: 3413837.
    Abstract:
    The barrier properties of reconstituted and native erythrocyte membranes frozen to -30, -40 or -70 degrees C and stored for a month were studied. The release of markers, namely haemoglobin molecules, [14C] sucrose and K+ ions from cells and membrane structures was measured. The main changes in the barrier function of ghosts and cells have been found to be due to freeze-thawing rather than to storage conditions. Glycerol, a cryoprotectant, appeared to stabilize the barrier properties of erythrocyte membranes for haemoglobin molecules, [14C] sucrose and to a lesser extent for K+ ions. The cryoprotective effect of glycerol has been shown to be considerably greater towards erythrocytes ghosts than to native erythrocytes.
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