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  • Title: An enzymatic histamine biosensor based on a screen-printed carbon electrode modified with a chitosan-gold nanoparticles composite cryogel on Prussian blue-coated multi-walled carbon nanotubes.
    Author: Nontipichet N, Khumngern S, Choosang J, Thavarungkul P, Kanatharana P, Numnuam A.
    Journal: Food Chem; 2021 Dec 01; 364():130396. PubMed ID: 34167007.
    Abstract:
    A histamine biosensor was developed based on a screen-printed carbon electrode modified with Prussian blue (PB) electrodeposited on multi-walled carbon nanotubes covered with a macroporous layer of chitosan-gold nanoparticles composite cryogel (CS-AuNPs Cry). With its high specific surface area and conductivity, CS-AuNPs Cry proved an excellent supporting material for diamine oxidase (DAO) immobilization. PB acted as a redox mediator to promote electron transfer between hydrogen peroxide and the electrode surface. The PB reduction current was measured during the hydrogen peroxide-releasing oxidation of histamine catalyzed by DAO. The proposed biosensor displayed two linear ranges: 2.50-125.0 µmol L-1 and 125.0-400.0 µmol L-1. The limit of detection was 1.81 µmol L-1. Reproducibility was good (RSD = 5.46%), operational stability high, long-term stability excellent, and selectivity good. The biosensor determined histamine levels in fish and shrimps with satisfactory recoveries, and the obtained results agreed with those obtained by ELISA.
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