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  • Title: Peptide-Based Biosensor with a Luminescent Copper-Based Metal-Organic Framework as an Electrochemiluminescence Emitter for Trypsin Assay.
    Author: Song X, Zhao L, Luo C, Ren X, Yang L, Wei Q.
    Journal: Anal Chem; 2021 Jul 20; 93(28):9704-9710. PubMed ID: 34242018.
    Abstract:
    A copper-based metal-organic framework (JUC-1000) has emerged as a promising electrochemiluminescence (ECL) emitter in the domains of bioanalysis and immunoassay. Herein, a highly efficient signal "on-off" peptide-based biosensor was constructed for trypsin (TPN) assay. JUC-1000 synthesized using an organic ligand of H4BDPO was functionalized as the ECL emitter, whose cathodic ECL behavior in aqueous media was first investigated using potassium persulfate (K2S2O8) as the coreactant. To further amplify the ECL signal, highly catalytic Ag@CeO2 nanoparticles were fabricated as both a substrate and an coreaction accelerator, which can efficiently catalyze the reduction of S2O82- to generate more sulfate anion radicals (SO4•-) for ECL enhancement, thereby generating strong and stable ECL signals in a "signal on" state. The functionalized JUC-1000 emitter was connected to the Ag@CeO2 sensing layer though a heptapeptide (HWRGWVC, HGC), and TPN as the target can specifically cleave the carboxyl side of arginine residues in HGC, leading to the release of emitters in a "signal off" state. Based on the efficient signal-switching, the biosensor exhibited linear ECL responses to the added TPN concentration, realizing sensitive detection of TPN in 10 fg/mL to 100 ng/mL with a limit of detection of 3.46 fg/mL. This work proposed an attractive orientation for the fundamental research of applying transition metal-organic frameworks as ECL emitters in bioanalysis and immunoassay.
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