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  • Title: Use of mild detergent gel electrophoresis for isolation and characterization of the kidney brush border D-glucose transporter.
    Author: Starita-Geribaldi M, Poiree JC, Sudaka P.
    Journal: Anal Biochem; 1987 Sep; 165(2):406-13. PubMed ID: 3425911.
    Abstract:
    Gradient gel electrophoresis was performed under mild detergent conditions to separate pig kidney brush border membrane proteins and to identify the smallest functional molecular protein entity of the D-glucose transporter. The various protein bands obtained from the nondenaturing gel system in a semipreparative scale were eluted by electrodialysis. These proteins were then reintegrated into proteoliposomes and tested for D-glucose-inhibitable [3H]phlorizin binding. The D-glucose transporter had a molecular mass of 70 kDa in mild detergent electrophoresis conditions and in subsequent SDS analysis.
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