These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Hsa_circ_0001326 regulates proliferation, migration, invasion, and EMT of HTR-8/SVneo cells via increasing IL16 expression.
    Author: Liu Y, Ma X, Liu Y.
    Journal: Am J Reprod Immunol; 2021 Nov; 86(5):e13484. PubMed ID: 34270857.
    Abstract:
    BACKGROUND: Preeclampsia (PE) is a serious pregnancy complication. It has been shown that insufficient infiltration of extravillous trophoblasts (EVTs) is related to the pathogenesis of PE. Circular hsa_circ_0001326 (circ_0001326) has been uncovered to be upregulated in PE. However, the influence of circ_0001326 on the infiltration of trophoblasts is indistinct. METHODS: 48 pregnant women (25 PE patients and 23 healthy controls) were recruited for this study. Human trophoblasts HTR-8/Svneo were used for function analysis. Expression of circ_0001326 was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR). The diagnostic value of circ_0001326 was analyzed by receiver operating characteristic (ROC) curve. Cell proliferation, migration, and invasion of HTR-8/Svneo cells were determined using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), 5-ethynyl-2'-deoxyuridine (EdU), wound-healing, and transwell assays. Protein levels were detected using Western blotting. The regulation mechanism of circ_0001326 was surveyed by bioinformatics analysis and dual-luciferase reporter assay. RESULTS: Circ_0001326 was highly expressed in plasma samples and placental tissues of PE patients. Moreover, plasma circ_0001326 could distinguish PE patients from healthy controls (area under the curve (AUC) =0.793). Functionally, circ_0001326 overexpression repressed HTR-8/Svneo cell proliferation, epithelial-mesenchymal transition (EMT), migration, and invasion, but circ_0001326 silencing had the opposing impact. Mechanically, circ_0001326 regulated interleukin-16 (IL16) expression via sponging microRNA (miR)-558. MiR-558 mimic or IL16 knockdown overturned the inhibiting effect of circ_0001326 overexpression on HTR-8/Svneo cell proliferation, EMT, migration, and invasion. CONCLUSION: Circ_0001326 elevated IL16 expression via sponging miR-558, thus curbing HTR-8/Svneo cell proliferation, EMT, migration, and invasion, suggesting that circ_0001326 might be involved in the pathogenesis of PE.
    [Abstract] [Full Text] [Related] [New Search]