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  • Title: Adhesion of Enteroaggregative E. coli Strains to HEK293 Cells.
    Author: Ayala-Lujan JL, Ruiz-Perez F.
    Journal: Bio Protoc; 2018 Apr 20; 8(8):e2802. PubMed ID: 34286021.
    Abstract:
    Enteroaggregative Escherichia coli (EAEC) is a recognized cause of acute diarrhea among both children and adults worldwide. EAEC strains are characterized by the presence of aggregative adherence fimbriae (AAF), which play a key role in pathogenesis by mediating attachment to the intestinal mucosa and by triggering host inflammatory responses. The aggregative adherence fimbria II (AAF/II) is the most important adherence factor of EAEC prototype strain 042 (EAEC042) to intestinal cells. Multiple receptors for AAF/II on epithelial cells have been identified including the transmembrane signaling mucin Muc1. This protocol describes a method to measure adherence of EAEC strains to HEK293 cells expressing the Muc1 glycoprotein.
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