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  • Title: Dome formation in cell cultures of chick embryo retinal pigment epithelium and effects of ouabain and 2,4-dinitrophenol thereon.
    Author: Kishida K, Kiritoshi A, Sasabe T, Matsumoto K, Manabe R.
    Journal: Jpn J Ophthalmol; 1987; 31(3):501-9. PubMed ID: 3430866.
    Abstract:
    Cells from chick embryo retinal pigment epithelium were cultured on glass slips. The primary culture cells formed confluent cell layers which were studded with a number of domes. The domes had usually appeared by the fourth day of culture and were susceptible to 3 X 10(-6) M ouabain and 10(-4) M 2,4-dinitrophenol, indicating that fluid was transepithelially transported from the apical to the basal side by means of an energy-requiring and ouabain-sensitive mechanism. Other than domes, small blisters appeared after 4-10 days of culture. They were also susceptible to the metabolic inhibitors.
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