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  • Title: [Characteristics of the structural organization of the cytoskeleton in suspension and monolayer sublines of mouse fibroblasts depending upon the type of growth in culture].
    Author: Bobrova IF, Komissarchik IaIu, Pinaev GP, Khomenko AV.
    Journal: Tsitologiia; 1987 Sep; 29(9):1007-11. PubMed ID: 3433357.
    Abstract:
    In attached cells, the main part of filaments is localized in submembrane area, whereas suspension fibroblasts are situated deeper in the cytoplasm. Another difference involves the form of filament gatherings. In the former these are bundles stretched parallel to the cell surface, whereas in the latter these gatherings are in the form of loose balls near the nucleus. According to their diameter and type of cell localization the latter filaments are similar to intermediate ones. In suspension cells with caps formed by ligand treatment, gatherings of fibrillar elements are observed within the cytoplasm but these filaments are shorter and looser, being located in disorder. In addition, the increase in the number of electron dense granules of unknown nature is observed between these filaments. The above observations suggest that the surface processes may be associated with the inner parts of cytoskeleton.
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