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  • Title: Effect of estradiol and progesterone on antistaphylococcal activity of neutrophils from ovariectomized mares.
    Author: Strzemienski PJ, Dyer RM, Kenney RM.
    Journal: Am J Vet Res; 1987 Nov; 48(11):1638-41. PubMed ID: 3434910.
    Abstract:
    Neutrophils isolated from jugular blood of ovariectomized mares were studied for the effect of estradiol and progesterone on bactericidal activity against Staphylococcus aureus. In experiment 1, neutrophils obtained from 4 mares were tested for bactericidal activity by adding estradiol (43 pg/ml) or progesterone (6.4 ng/ml) to the bactericidal assay. In experiment 2, 3 of the 4 ovariectomized mares were given 2 mg of estradiol, IM, daily for 3 days. Eighteen days after the initial estradiol injection, mares were given 300 mg of progesterone, IM, for 6 days. Neutrophils from these mares were tested for bactericidal activity 4 days after the initial estradiol injection, 17 days after the initial estradiol injection (control), and 7 days after the first progesterone injection. Bactericidal activity was measured at 30 and 120 minutes by counting the number of colony-forming units remaining. Neutrophil antistaphylococcal activity was not altered by adding estradiol and progesterone to the assay or by supplementing ovariectomized mares with estradiol and progesterone (P greater than 0.05).
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