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  • Title: [Human extracorporeal fertilization with subsequent successful embryo implantation and the birth of a child].
    Author: Nikitin AI, Kitaev EM, Savitskiĭ GA, Ivanova RD, Kalashnikova EP.
    Journal: Arkh Anat Gistol Embriol; 1987 Oct; 93(10):39-43. PubMed ID: 3435254.
    Abstract:
    With the aim to use a previously elaborated method of extracorporeal fertilization of human ova for treating sterility, resulted from obstruction or absence of the uterine tubes, ova were obtained at laparoscopy after a previous hormonal stimulation of women. From 119 women 159 ova were obtained. Fertilization of the ova was performed in the medium Ham F-10 previously capacitated with the husband's spermatozoa. Fertilization rate was 66%. Embryos at the stage of 2-8 blastomeres were implanted transcervically into the uterine cavity with a special catheter. The embryo transfer was performed in 37 women with tubular infecundity. Menoschesis up to 3 weeks was noted in 5 women. In one woman on the 35th day after implantation, by means of hormonal-biological reaction and ultrasound investigation, pregnancy was diagnosed, it terminated in timely birth of a boy with body mass 3,500 gr. A conclusion was made that the main cause of failures at the embryo transfer was an inadequate preparation of the uterine mucosa (resulted from the main disease) to accept the fertilized ovum.
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