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Title: [Effect of electroacupuncture on 5-HT7R in gastric antrum and colon tissues of functional diarrhea rats].
Author: Wang XY, Li CY, Zhang YN, Fu CY, Gao SS, Wang SJ.
Journal: Zhen Ci Yan Jiu; 2021 Jul 25; 46(7):549-54. PubMed ID: 34369673.
Abstract:
OBJECTIVE: To observe the effect of electroacupuncture(EA) at "Zusanli"(ST36), "Yinlingquan" (SP9) or "Yingu"(KI10) on the expression of 5-hydroxytryptamine type 7 receptor (5-HT7R) in the gastric antrum and colon tissues in functional diarrhea (FD) model rats, so as to explore its mechanisms underlying improving FD. METHODS: Forty male SD rats were randomly divided into control, model, ST36, SP9 and KI10 groups，with 8 rats in each group. The FD model was established by combined administration of restriction (four-limbs' banding) + abdominal cold stimulation + feeding every other day, for 14 days. EA (2 Hz, 0.5 mA) was applied to bilateral ST36 or bilateral SP9 or bilateral KI10 in the 3 corresponding groups for 30 min, once a day for 7 days after successful modeling. Rats of the control group received restriction only. The fecal water content was calculated and the stool form score was given according to the Bristol's methods. The gastric residual rate (GRR) and small intestine propulsion rate (SIPR) were determined to assess the motility of the gastrointestinal tract. Immunohistochemical and real-time fluorescent quantitative PCR were used to detect the expression of 5-HT7R protein and mRNA of the gastric antrum and colon tissues, respectively. RESULTS: Compared with the control group, the fecal water content, the stool form score, the SIPR and the expression levels of 5-HT7R protein and 5-HT7R mRNA were significantly increased (P<0.01,P<0.05) and the GRR was considerably decreased in the model group (P<0.01). The fecal water content, stool form score and SIPR, and expression level of 5-HT7R protein and mRNA in the gastric antrum and colon were significantly lower in both the ST36 and SP9 groups (not in the KI10 group) than in the model group (P<0.01, P<0.05), but the GRR was significantly higher in the ST36 and SP9 groups (not in the KI10 group) than in the model group (P<0.01). The effects of both ST36 and SP9 were significantly superior to those of KI10 in improving all the indexes mentioned above （except SIPR and the mRNA level of 5-HT7R in the colon in SP9 group）(P<0.01, P<0.05). No significant differences were found between the ST36 and SP9 groups in lowering the levels of fecal water content, stool form score, SIPR, and the expression of 5-HT7R protein and mRNA, as well as in up-regulating GRR (P>0.05). CONCLUSION: EA of ST36 and SP9 can improve the motility of gastrointestinal tract in FD rats, which may be related to its functions in down-regulating the expression of 5-HT7R protein and mRNA in gastric antrum and colon tissues. The effects of ST36 and SP9 were obviously better than those of KI10 in ameliorating the gastric and intestinal motility (except GRR) and in lowering the expression of 5-HT7R protein and mRNA. 目的：观察电针不同穴位对功能性腹泻(FD)模型大鼠胃窦、结肠中五羟色胺7受体(5-HT7R)表达的影响，探讨电针对FD大鼠胃肠运动的调节机制及经穴脏腑相关性。方法：将40只雄性SD大鼠随机分为正常组、模型组、足三里组、阴陵泉组、阴谷组，每组8只。连续14 d用束缚+冷刺激+饮食失节的复合病因法制备FD大鼠模型。模型复制成功后，足三里组、阴陵泉组、阴谷组大鼠给予相应穴位电针干预30 min，强度0.5 mA，1次/d，共7 d。计算各组大鼠粪便含水量并按Bristol分级进行粪便性状评分，测定胃内残留率、小肠推进率，并采用免疫组织化学法检测胃窦、结肠中5-HT7R蛋白的表达，实时荧光定量PCR法检测胃窦、结肠中5-HT7R mRNA的表达。结果：与正常组比较，模型组大鼠粪便含水量、粪便性状评分、小肠推进率均明显升高(P<0.01)，胃内残留率降低(P<0.01)，胃窦、结肠组织中5-HT7R蛋白和mRNA表达升高(P<0.05)。与模型组比较，足三里组、阴陵泉组大鼠粪便含水量、粪便性状评分、小肠推进率均明显降低(P<0.01，P<0.05)，胃内残留率明显升高(P<0.01)，胃窦与结肠中5-HT7R蛋白和mRNA表达明显降低(P<0.05)。与阴谷组比较，足三里组、阴陵泉组大鼠粪便含水量、粪便性状评分均明显降低(P<0.01，P<0.05)，胃内残留率升高(P<0.01)，胃窦中5-HT7R蛋白和mRNA、结肠中5-HT7R蛋白表达降低(P<0.05)；足三里组小肠推进率降低(P<0.05)，结肠中5-HT7R mRNA表达降低（P<0.05）。结论：电针“足三里”“阴陵泉”能改善FD模型大鼠的症状，其机制可能与降低胃窦、结肠组织的5-HT7R蛋白和mRNA的表达有关，且该作用具有一定的穴位特异性。.

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