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  • Title: Cryopreservation of human oocytes.
    Author: Al-Hasani S, Diedrich K, van der Ven H, Reinecke A, Hartje M, Krebs D.
    Journal: Hum Reprod; 1987 Nov; 2(8):695-700. PubMed ID: 3437048.
    Abstract:
    Three different methods were used for freezing human excess oocytes (320 frozen, 205 thawed) in our IVF programme and the results of these methods were compared. A high fertilization rate (75%) could be achieved after thawing, using 1,2 propanediol as a cryoprotectant. Polyploidy rates of 20% and 40% were observed using DMSO and 1,2-propanediol as cryoprotectants, respectively. Using the ultracooling method, the survival rate was poor (4%).
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