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  • Title: A kinetic method for quantification of aspartate aminotransferase isoenzymes.
    Author: Gracia R, Busquets M, Gil M, Cortés A, Bozal J.
    Journal: J Biochem Biophys Methods; 1987 Nov; 15(2):63-9. PubMed ID: 3437099.
    Abstract:
    A spectrophotometric assay is proposed to determine the levels of aspartate aminotransferase (AAT) isoenzymes from chicken liver by a steady-state kinetic method which depends on the differential inhibition of these isoenzyme forms by high concentrations of substrate 2-oxoglutarate at pH 6.2. The use of a standard curve permits the determination of the percentage of chicken liver c-AAT and m-AAT isoenzymes. This method yields results in good correlation with those achieved by different extent adipate inhibition and by differential centrifugation.
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