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  • Title: Discovery of Polycyclic Macrolide Shuangdaolides by Heterologous Expression of a Cryptic trans-AT PKS Gene Cluster.
    Author: Liu Y, Zhou H, Shen Q, Dai G, Yan F, Li X, Ren X, Sun Q, Tang YJ, Zhang Y, Bian X.
    Journal: Org Lett; 2021 Sep 03; 23(17):6967-6971. PubMed ID: 34388000.
    Abstract:
    A cryptic trans-acyltransferase polyketide synthase biosynthetic gene cluster sdl (80 kb) from Streptomyces sp. B59 was cloned and transferred into a heterologous host Streptomyces albus J1074, resulting in a class of polycyclic macrolide shuangdaolides A-D (1-4) and dumulmycin (5). Heterologous expression and gene inactivation experiments allowed the identification of two biosynthetic intermediates, 6 and 7, suggesting an unusual multidomain SDR oxidoreductase SdlR in charge of the formation of a rare 2-hydroxycyclopentenone moiety in this class of compounds.
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