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  • Title: A novel peptide-based fluorescent probe with a large stokes shift for rapid and sequential detection of Cu2+ and CN- in aqueous systems and live cells.
    Author: Guo Z, Wang Q, Zhou D, An Y, Wang P, Liao F.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2022 Jan 05; 264():120257. PubMed ID: 34411770.
    Abstract:
    A novel fluorescent probe (DSD) was reasonably designed and synthesized with dansyl-labeled dipeptide (Dan-Ser-Asp-NH2). DSD featured remarkably large Stokes shift (230 nm) and perfect water solubility, and exhibited high selectivity and rapid recognition toward Cu2+via fluorescence quenching. The detection limit of DSD for Cu2+ was 2.4 nM, indicated that DSD has excellent sensitivity. In addition, the stoichiometry between DSD and Cu2+ were detected as 1:1 by fluorescence titration, Job's plot and ESI-HRMS data. As designed, DSD-Cu2+ system was able to sequentially detect CN- according to the displacement approach with fluorescence "off-on" response, and the detection limit for CN- was calculated to be 41.9 nM. Specifically, the response time of DSD with Cu2+ and CN- was less than 40 s, which rendered it suitable for real time detection in actual water samples. In addition, with the alternate addition of Cu2+ and CN-, the reversible cycles could be repeated for at least 10 times, indicated that DSD was a promising reversibility probe. DSD showed low toxicity and good biocompatibility, and was successfully applied to detect Cu2+ and CN- in living cells.
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