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  • Title: A Mycoplasma gallisepticum strain-specific DNA probe.
    Author: Khan MI, Kirkpatrick BC, Yamamoto R.
    Journal: Avian Dis; 1987; 31(4):907-9. PubMed ID: 3442542.
    Abstract:
    Total DNA from the vaccine F strain (K810) and the reference S6-strain of Mycoplasma gallisepticum (MG) was cloned in Escherichia coli using the plasmid pUC8. A 6-kilobase fragment, specific for the vaccine strain, was identified by colony dot and Southern hybridization analyses. When labeled and used as a probe, this fragment hybridized with the homologous and one other vaccine F-strain (F2F10), but it did not hybridize with other MG strains (Fg38, S6, A5969, V503) or with three other species of avian mycoplasmas.
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