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  • Title: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA.
    Author: Mohammadniaei M, Zhang M, Ashley J, Christensen UB, Friis-Hansen LJ, Gregersen R, Lisby JG, Benfield TL, Nielsen FE, Henning Rasmussen J, Pedersen EB, Olinger ACR, Kolding LT, Naseri M, Zheng T, Wang W, Gorodkin J, Sun Y.
    Journal: Nat Commun; 2021 Aug 24; 12(1):5089. PubMed ID: 34429424.
    Abstract:
    The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL-1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.
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