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  • Title: Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit.
    Author: Horova V, Landova B, Hodek J, Chalupsky K, Krafcikova P, Chalupska D, Duchoslav V, Weber J, Boura E, Klima M.
    Journal: Viruses; 2021 Jul 29; 13(8):. PubMed ID: 34452352.
    Abstract:
    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2'-O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2'-O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
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