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  • Title: [Effect of electroacupuncture of "Zhiyang" (GV9) and "Jizhong" (GV6) on expression of CGRP and NLRP3 in rats with spinal cord injury].
    Author: Guo M, Xiang JQ, Wei LH, Cui YJ.
    Journal: Zhen Ci Yan Jiu; 2021 Aug 25; 46(8):679-83. PubMed ID: 34472753.
    Abstract:
    OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Zhiyang" (GV9) and "Jizhong" (GV6) of the Governor Vessel on the expression of calcitonin gene related peptide (CGRP)and NOD-like receptor pyrin containing 3 (NLRP3) in the injured anterior horn (AH) of rats with spinal cord injury (SCI), so as to explore its mechanisms underlying improvement of SCI. METHODS: Thirty-six male SD rats were randomly divided into sham operation (sham) group, model group and EA group which were further randomly allocated to 7 day (d) and 14 d subgroups (n=6 per subgroup). The SCI model was established by clamping the exposed spinal cord with an aneurysm clip. Rats of the EA group received EA of GV6 and GV9 for 30 min, once daily for 7 and 14 days, respectively. The Basso, Beattie and Bresnahan (BBB) was used to assess changes of the locomotor function on the 1st, 3rd, 7th and 14th day after SCI. The protein expression levels of CGRP, NLRP3, apoptosis-associated speck-like protein (ASC) and cysteinyl aspartate specific protease-1 (caspase-1) in the anterior horn region of the spinal cord on the 7th and 14th after SCI were detected by Western blot. RESULTS: The BBB scores on the 1st, 3rd, 7th and 14th d after SCI were significantly lower in the model group than in the sham group (P<0.05), whereas the expression levels of NLRP3, ASC and caspase-1 proteins on day 7 and 14 were markedly higher in the model group than in the sham group (P<0.05, P<0.01). Compared with the model group, the BBB score and CGRP expression on the 7th and 14th d were significantly increased in the EA group (P<0.05,P<0.01), while the expression levels of NLRP3, ASC and caspase-1 were obviously decreased in the EA group (P<0.01). CONCLUSION: EA of GV6 and GV9 can improve the locomotion of SCI rats, which may be associated with its function in up-regulating the expression of CGRP and down-regulating the expression of NLRP3, ASC and caspase-1 proteins in the spinal anterior horn tissue. 目的：观察电针督脉“至阳”“脊中”对脊髓损伤(SCI)大鼠脊髓损伤区降钙素基因相关肽(CGRP)和Nod样受体蛋白3(NLRP3)炎性小体表达的影响，探讨电针对脊髓损伤的保护作用机制。方法：将36只雄性SD大鼠随机分为假手术组、模型组和电针组，每组再随机分为7 d组和14 d组两个亚组，每组6只。采用钳夹法复制SCI大鼠模型。电针组予电针刺激“至阳”“脊中”，每次30 min，分别连续治疗7 d或14 d。采用BBB行为学评分法观察术后1、3、7、14 d大鼠运动功能的变化，采用蛋白免疫印记法检测术后7 d和14 d脊髓损伤处CGRP、NLRP3、凋亡相关的斑点样蛋白(ASC)和半胱氨酸天冬氨酸蛋白酶-1(caspase-1)的蛋白表达水平。结果：与同时点假手术组比较，模型组的BBB评分降低(P<0.05)；与同时点模型组比较，电针组术后7、14 d的BBB评分升高(P<0.05)。与同时点假手术组比较，模型组术后7、14 d脊髓损伤处NLRP3、ASC和caspase-1的蛋白表达均升高(P<0.05，P<0.01)；与同时点模型组比较，电针组术后7、14 d脊髓损伤处NLRP3、ASC和caspase-1的蛋白表达均降低(P<0.01)，CGRP蛋白表达均升高(P<0.01)。结论：电针督脉可明显改善SCI大鼠的运动功能，其机制可能与上调CGRP的表达，抑制NLRP3炎性小体的活化有关，CGRP可能是电针抑制NLRP3启动的上游因子。.
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