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  • Title: The complete primary structure of the rat A-raf cDNA coding region: conservation of the putative regulatory regions present in rat c-raf.
    Author: Ishikawa F, Takaku F, Nagao M, Sugimura T.
    Journal: Oncogene Res; 1987 Aug; 1(3):243-53. PubMed ID: 3449797.
    Abstract:
    A-raf gene was first detected by low-stringent hybridization with v-raf and was reported as a possible oncogene. We have cloned rat A-raf cDNA and determined the complete nucleotide sequence over its long open reading frame. Alignment of rat A-raf- and c-raf-deduced amino acid sequences shows 59.4% homology. Both the ATP binding site and the kinase domain are conserved in A-raf as in c-raf. Furthermore, three regions highly conserved between A-raf and c-raf were identified in the 5'-half region of c-raf which has been shown to be deleted in the activated form of c-raf. These regions were speculated to be the candidates of the regulatory domain controlling the protein kinase activity of raf products. Southern blot analysis shows that genes homologous to A-raf and c-raf are present as independent loci in Drosophila melanogaster, Caenorhabditis elegans, Saccharomyces cerevisiae and Candida boidinii. A-raf was expressed in several tissues and cell line at various amounts.
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