These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A rapid method for the isolation of circular DNA using an aqueous two-phase partition system.
    Author: Ohlsson R, Hentschel CC, Williams JG.
    Journal: Nucleic Acids Res; 1978 Feb; 5(2):583-90. PubMed ID: 345246.
    Abstract:
    A method of isolating circular plasmid DNA from cleared lysates of E. coli is described. Purification is achieved by virtue of the rapid re-annealing kinetics or supercoiled DNA. After a brief denaturation step, double stranded plasmid DNA is separated from denatured chromosomal DNA and RNA in a two-phase partition system using dextran and polyethylene glycol. The method is much more rapid than the conventional dye-centrifugation technique and plasmid DNA of comparable purity and yield is obtained.
    [Abstract] [Full Text] [Related] [New Search]