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  • Title: A non-linear regression program in BASIC for estimating Km and Vmax.
    Author: Page A.
    Journal: Comput Appl Biosci; 1987 Mar; 3(1):49-51. PubMed ID: 3453214.
    Abstract:
    This paper gives a program in BASIC for calculating the kinetic parameters Km and Vmax for an enzyme reaction from a set of paired values of reaction velocity at given substrate concentrations. An initial estimate of the two parameters is made using a weighted linear regression, these values are then used in an iterative process to fit the data to the Michaelis-Menten equation and give final values of Km and Vmax with their associated standard errors.
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