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  • Title: Isolation of pathogen-synthesized fraction that elicits phytoalexins in lucerne (Medicago sativa L.).
    Author: Onuorah OM.
    Journal: Acta Biol Hung; 1987; 38(2):247-56. PubMed ID: 3454086.
    Abstract:
    The production of phytoalexins by lucerne cultivars Dupuits (susceptible to Verticillium wilt) and Maris Kabul (resistant to Verticillum wilt) in response to substances (= elicitors) produced by pathogenic and non-pathogenic isolates of Verticillium albo-atrum was investigated. The elicitors are produced in culture filtrates from log-phase and past log-phase stages. The elicitors were isolated by gel-filtration and found to contain both protein and carbohydrate. They were found to be thermostable and non-dialyzable and proved to be sensitive to protease and sodium hydroxide treatment which suggests that they are glycoproteins. There was no evidence of specificity of the elicitors.
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