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  • Title: Combination of hydrothermal and chemi-mechanical pretreatments to enhance enzymatic hydrolysis of poplar branches and insights on cellulase adsorption.
    Author: Wu R, Liu W, Li L, Ren Q, Jiang C, Hou Q.
    Journal: Bioresour Technol; 2021 Dec; 342():126024. PubMed ID: 34600090.
    Abstract:
    An integration of different pretreatments is important to overcome recalcitrance and realize efficient bioconversion of lignocellulosic biomass. This study aims at the effects of combination of hydrothermal pretreatment and different chemi-mechanical pretreatments on enzymatic hydrolysis, and understanding the enzymes adsorption mechanism. The combination of hydrothermal and chemi-mechanical pretreatments effectively improved the enzymatic hydrolysis of poplar substrates, in which the enzymatic hydrolysis of substrates pretreated by hydrothermal pretreatment + Fenton pretreatment + mechanical refining (HFM) was the highest (92.39% of glucose conversion yield, and 20.88 g/L of glucose concentration). The substrates' main characteristics were obviously changed after combined pretreatments, such as swelling ability and specific surface area of substrates were increased. The Langmuir adsorption model (R2 > 0.98) and pseudo second-order adsorption kinetic model (R2≈1) were well suitable to describe the adsorption of enzymes on substrates, meanwhile the adsorption mechanism was summarized.
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