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Title: [Effect of moxibustion on colonic mucosal injury and expression of CGRP positive nerve fibers of distal colonic mucosa in ulcerative colitis mice].
Author: Huang R, Wu JH, Wang SL.
Journal: Zhongguo Zhen Jiu; 2021 Oct 12; 41(10):1127-34. PubMed ID: 34628746.
Abstract:
OBJECTIVE: To observe the effect of moxibustion at "Zusanli" (ST 36) on distal, middle and proximal colonic mucosal injury and expression of calcitonin gene-related peptide (CGRP) positive nerve fibers of distal colonic mucosa in ulcerative colitis (UC) mice at different time points. METHODS: A total of 51 C57BL/6N mice were randomized into a 7-day control group (n=8), a 7-day model group (n=7), a 7-day moxibustion group (n=7), a 14-day control group (n=6), a 14-day model group (n=14) and a 14-day moxibustion group (n=9). In the model groups and the moxibustion groups, 2% dextran sulfate sodium (DSS) was given for 7-day free drinking to establish the UC model. Three days into modeling, moxibustion was applied at "Zusanli" (ST 36) in the 7-day moxibustion group and the 14-day moxibustion group, once a day, 10 min a time for 5 days and 12 days respectively. HE staining was used to observe the morphology of colonic tissue, the percentages of distal, middle and proximal colonic mucosal injury were calculated. Immunofluorescence staining was used to detected the expressions of positive nerve fibers of distal, middle and proximal colonic mucosa and CGRP positive nerve fibers of distal colonic mucosa. RESULTS: Mucosal injury can be observed in mice after modeling, displaying epithelial layer disappearance, abnormal crypt structure or crypt disappearance. Compared with the 7-day control group, colon length was shortened (P<0.001), percentages of overall, distal, middle colonic mucosal injury were increased (P<0.001), the expressions of positive nerve fibers of distal, middle and proximal colonic mucosa and CGRP positive nerve fibers of distal colonic mucosa were increased (P<0.001, P<0.05, P<0.01) in the 7-day model group. Compared with the 7-day model group, the expressions of positive nerve fibers of middle and distal colonic mucosa and CGRP positive nerve fibers of distal colonic mucosa were decreased in the 7-day moxibustion group (P<0.05). Compared with the 14-day control group, the colon length was shortened (P<0.01), percentage of overall colonic mucosal injury was increased (P<0.001) in the 14-day model group. Compared with the 14-day model group, colon length was lengthened (P<0.05), percentage of overall colonic mucosal injury was decreased (P<0.05) in the 14-day moxibustion group. CONCLUSION: Moxibustion at "Zusanli" (ST 36) can reduce the expressions of positive nerve fibers of colonic mucosa and CGRP positive nerve fibers of distal colonic mucosa, thus, improve the colonic mucosal injury. 目的：观察艾灸“足三里”对溃疡性结肠炎（UC）模型小鼠不同时间段结肠远端、中段、近端黏膜损伤及结肠远端黏膜层降钙素基因相关肽（CGRP）阳性神经纤维表达的影响。方法：将51只C57BL/6N小鼠随机分为7 d对照组（8只）、7 d模型组（7只）、7 d艾灸组（7只）、14 d对照组（6只）、14 d模型组（14只）和14 d艾灸组（9只）。模型组和艾灸组均予2%葡聚糖硫酸钠（DSS）溶液自由饮用7 d建立UC模型。造模3 d后，7 d艾灸组及14 d艾灸组予艾灸“足三里”干预，每天1次，每次10 min，分别干预5、12 d。采用HE染色法观察各组小鼠结肠组织形态，计算结肠远端、中段、近端黏膜损伤长度百分比。采用免疫荧光法观察各组小鼠结肠远端、中段、近端损伤部位黏膜层神经纤维及结肠远端黏膜层CGRP阳性神经纤维的表达。结果：造模后小鼠出现多处黏膜损伤，表现为上皮层消失、隐窝结构异常或消失。与7 d对照组比较，7 d模型组小鼠结肠长度更短（P<0.001），结肠全段、远端及中段黏膜损伤长度百分比增加（P<0.001）；结肠远端、中段、近端损伤部位黏膜层神经纤维阳性表达及远端黏膜层CGRP阳性神经纤维表达增加（P<0.001，P<0.05，P<0.01）。与7 d模型组比较，7 d艾灸组小鼠结肠远端及中段损伤部位黏膜层神经纤维阳性表达及远端黏膜层CGRP阳性神经纤维表达减少（P<0.05）。与14 d对照组比较，14 d模型组小鼠结肠长度更短（P<0.01），结肠全段黏膜损伤长度百分比增加（P<0.001）。与14 d模型组比较，14 d艾灸组小鼠结肠长度更长（P<0.05），结肠全段黏膜损伤长度百分比减少（P<0.05）。结论：艾灸“足三里”可减少UC模型小鼠结肠黏膜层神经纤维及CGRP阳性神经纤维表达，从而改善结肠黏膜损伤程度。.

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