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  • Title: Localization of Chinese hamster dihydrofolate reductase gene to band p23 of chromosome 2.
    Author: Funanage VL, Myoda TT.
    Journal: Somat Cell Mol Genet; 1986 Nov; 12(6):649-55. PubMed ID: 3466362.
    Abstract:
    It has been shown that gamma irradiation causes extensive deletions at the dihydrofolate reductase (dhfr) locus in Chinese hamster ovary (CHO) cells. We have analyzed seventeen DHFR-negative (DHFR-) mutants of CHO cells for cytogenetic alterations involving the dhfr locus on chromosome 2. Five DHFR- mutants contained the same large deletion [del(2)(p16p23)] in the short arm of chromosome 2. This deletion comprised about 18% of the short arm and was estimated to be 41,000 kb in length. Four other DHFR- mutants contained smaller deletions of about 9200 kb. One of these mutants had a partial deletion of bands 2p22 and 2p23, whereas the others showed deletion of band 2p23. Inversions of chromosome 2 were seen in two other DHFR- mutants. An analysis of the breakpoints involved in these cytogenetic alterations indicates that the hamster dhfr gene resides in band p23 of chromosome number 2.
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