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  • Title: Bio-Specific Au/Fe3+ Porous Spongy Nanoclusters for Sensitive SERS Detection of Escherichia coli O157:H7.
    Author: Li Y, Gao F, Lu C, Fauconnier ML, Zheng J.
    Journal: Biosensors (Basel); 2021 Sep 24; 11(10):. PubMed ID: 34677310.
    Abstract:
    For sensitive and fast detection of Escherichia coli O157:H7, organic and inorganic hybrid Au/Fe3+ nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe3+ porous spongy NCs with large surface area showed excellent bio-specific capability for E. coli O157:H7. GNPs in Au/Fe3+ NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe3+ NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of E. coli O157:H7, which showed a good linear response (101 to 106 cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60-97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of E. coli O157:H7.
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