MEDLINE/PubMed Journal Browser Search

Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

Title: [Effect of electroacupuncture stimulation of different acupoint groups on lipid metabolism and liver TLR4/NF-κB signaling in obese rats].
Author: Yang SR, Zhou YD, Chen R, Lu W, Wu S, Wang YY, Chen L, Liang FX.
Journal: Zhen Ci Yan Jiu; 2021 Oct 25; 46(10):845-50. PubMed ID: 34698458.
Abstract:
OBJECTIVE: To observe the effect of electroacupuncture (EA) of different acupoint combinations on lipid metabolism and liver Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling in obese rats, so as to explore the specificity of regulatory effects of different acupoints for obesity. METHODS: Male SD rats were randomly divided into normal control, model, hindlimb acupoint EA ［"Fenglong" (ST40)-"Zusanli" (ST36), hindlimb EA］, abdominal acupoint EA ［("Zhongwan" (CV12)-"Tianshu" (ST25), "Guanyuan" (CV4) - the opposite ST25, abdominal EA］, BiaoBen-acupoint EA ［ST40-ST36, CV12 -CV4, ST25, BiaoBen EA］ groups, with 10 rats in each group. The obesity model was induced by feeding the rats with high fat diet. EA (2 Hz, 1 mA) was applied to the acupoint groups mentioned above for 10 min (per acupoint group), 3 times a week for 8 weeks. After the treatment, the body weight and food intake in each group were recorded. The contents of serum total cholesterol (TC), triglyceride (TG), non-esterified fatty acid (NEFA) were detected by using an automatic biochemical analyzer. The expression levels of TLR4 and NF-κB p65 mRNAs and proteins in the liver tissue were detected by quantitative real-time PCR and Western blot, separately. RESULTS: Compared with the normal group, the body weight and food intake, serum TC, TG and NEFA contents, hepatic TLR4 and NF-κB p65 protein and gene expression were significantly increased in the model group (P<0.05, P<0.01). After the treatment, compared with the model group, the body weight, food intake, TC, TG, NEFA, TLR4 and NF-κB p65 protein and mRNA expression were significantly decreased in the hindlimb EA, abdominal EA and BiaoBen EA groups (P<0.05, P<0.01). Comparison among the 3 treatment groups showed that the serum TC, TG and NEFA contents were significantly lower in the hindlimb EA and BiaoBen EA groups than in the abdominal EA group (P<0.01，P<0.05). The expression levels of TLR4 and NF-κB p65 mRNAs and proteins were significantly lower in the BiaoBen EA group than in the hindlimb EA and abdominal EA groups (P<0.01, P<0.05). CONCLUSION: BiaoBen EA is superior to abdominal EA in improving lipid metabolism in obesity rats, which may be related to its stronger effectiveness in down-regulating hepatic TLR4/NF-κB p65 signaling. 目的：观察不同腧穴配伍电针对肥胖大鼠脂代谢和肝脏Toll样受体4(TLR4)/核转录因子κB (NF-κB)信号通路的影响，探讨不同腧穴配伍电针对肥胖的调节效应是否具有差异性。方法：Wistar大鼠随机分为正常组、模型组、下肢电针组、腹部电针组、标本配穴组，每组10只。采用高脂饮食喂养诱导肥胖大鼠模型。下肢电针组选取“足三里”“丰隆”，腹部电针组选取“中脘”“天枢”“关元”，标本配穴组选取上述所有腧穴，分别给予电针治疗，10 min/次，3次/周，共治疗8周。治疗后，观察各组大鼠体质量、进食量，全自动生化分析仪检测各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(NEFA)含量，Western blot法检测大鼠肝脏组织TLR4、NF-κB p65蛋白表达，实时荧光定量PCR法检测大鼠肝脏组织TLR4、NF-κB p65 mRNA表达。结果：与正常组比较，模型组大鼠体质量、进食量、血清脂代谢指标（TC、TG、NEFA）及肝脏TLR4、NF-κB p65蛋白和mRNA表达均升高(P<0.05, P<0.01)。治疗后，与模型组比较，3个干预组体质量、进食量、血清脂代谢指标（TC、TG、NEFA）及肝脏TLR4、NF-κB p65 蛋白和mRNA表达降低(P<0.05, P<0.01)。与下肢电针组比较，腹部电针组血清TC、TG、NEFA升高(P<0.01, P<0.05)；肝脏TLR4 mRNA表达降低(P<0.05)；标本配穴组肝脏TLR4、NF-κB p65蛋白和mRNA表达降低(P<0.01, P<0.05)。与腹部电针组比较，标本配穴组血清TC、TG、NEFA及肝脏TLR4、NF-κB p65蛋白表达和NF-κB p65 mRNA表达降低(P<0.01, P<0.05)。结论：标本配穴电针和下肢电针对血脂的调节作用优于腹部电针，这可能与“足三里”“丰隆”穴对脂代谢相关基因的调控有关；不同腧穴配伍电针对肝脏TLR4、NF-κB p65调节作用的差异可能与对炎性相关信号因子的调控有关。.

[Abstract] [Full Text] [Related] [New Search]