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  • Title: [Measurement of total bile acids. Comparison of different preparations of 3 alpha-hydroxysteroid dehydrogenase].
    Author: Feldmann D, Beaudonnet A, Goudable J, Petigny B, Sarmini H, Vercambre C.
    Journal: Ann Biol Clin (Paris); 1987; 45(1):47-51. PubMed ID: 3472477.
    Abstract:
    Oxidation of free and conjugated biliary acids was carried out with 5 different enzymatic preparations of 3 alpha-hydroxysteroid dehydrogenase. The comparison of the catalytic activities obtained, shows the existence of important variations according to the enzymes. Preparations purified by chromatography have a stronger activity toward primary biliary acids than toward secondary biliary acids, while preparations obtained from bacterial mutants have a low activity toward cholic acid and its conjugates.
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