These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Physical and genetic analysis of cosmids from the vicinity of the cystic fibrosis locus.
    Author: Scambler PJ, Estivill X, Bell G, Farrall M, McLean C, Newman R, Little PF, Frederick P, Hawley K, Wainwright BJ.
    Journal: Nucleic Acids Res; 1987 May 11; 15(9):3639-52. PubMed ID: 3473444.
    Abstract:
    Cosmid libraries have been constructed from DNA of somatic cell hybrid cell lines, each containing a fragment of human chromosome seven and including sequences closely linked to cystic fibrosis (CF). Cosmids containing human DNA as insert were isolated from the library. Three cosmids, when used as probes to total genomic DNA, detected polymorphic loci, each of which was shown to be in strong linkage disequilibrium with CF. Restriction endonuclease digestion of cosmid clones and use of a new, rapid method of chromosome walking based on competitive hybridisation of cosmid inserts has allowed identification of several groups of overlapping cosmids ("contigs") from the vicinity of CF.
    [Abstract] [Full Text] [Related] [New Search]