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  • Title: A novel aptamer RRS assay platform for ultratrace melamine based on COF-loaded Pd nanocluster catalytic amplification.
    Author: Wang H, Zhao Y, Shi J, Wen G, Liang A, Jiang Z.
    Journal: J Hazard Mater; 2022 Feb 05; 423(Pt B):127263. PubMed ID: 34844371.
    Abstract:
    Two COFs of BzBD and BzBD loaded Pd nanoclusters (BzBDPd) were prepared using 1,3,5-benzenetricarboxaldehyde (Bz), benzidine (BD) and CO reducing agent, and were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), infrared spectroscopy (IR) and other techniques. BzBDPd can strongly catalyze the new and stable Au@NiP nanoreaction that exhibit a strong resonance Rayleigh scattering (RRS) peak at 538 nm and a surface plasmon resonance (SPR) absorption peak at 395 nm, and the sensitive and facile RRS technique was used to study the indicator reaction. Combining the nanocatalytic amplification reaction with specific aptamer (Apt) of some target molecules such as melamine (ML), urea (UR) and bisphenol A (BPA), a simple, sensitive and selective Apt RRS assay platform was established. The linear range of the RRS detection platform for melamine is 0.0025-0.04 nmol/L, and the detection limit (DL) is 1.96 × 10-4 nmol/L. In addition, ML in real sample was analyzed, the stability of BzBD, BzBDPd, PdNPs and the catalytic mechanism of COFPd were also considered.
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