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  • Title: Comparative study of the effect of serum containing granulocyte-stimulating activity and colony-stimulating activity on hematopoiesis in triple-set diffusion chambers.
    Author: Jovcić G, Stojanović N, Biljanović-Paunović L, Hajduković S.
    Journal: Exp Hematol; 1986 May; 14(4):298-302. PubMed ID: 3486132.
    Abstract:
    Serum obtained from rats with polyvinylpyrrolidone-induced sterile inflammation contains granulopoiesis-stimulating activity (GSA). In triple-set diffusion chambers (DC) with GSA in the middle chamber a stimulation of morphologically recognizable granulocytic cells (MRG) was found in mouse bone marrow cells cultivated in side chambers for 6 and 24 h. GSA also induced an increase in the number of CFU-GM when DC were inplanted into the peritoneum of normal rats. Under the same experimental conditions, colony-stimulating factor (CSA) from mouse-lung-conditioned medium did not cause such changes either in MRG or in CFU-GM compartments. The data obtained indicate the different stimulating action of GSA and CSA on granulopoiesis.
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