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  • Title: Exploring the Diversity of the B-Cell Receptor Repertoire Through High-Throughput Sequencing.
    Author: Hom JR, Tomar D, Tipton CM.
    Journal: Methods Mol Biol; 2022; 2421():231-241. PubMed ID: 34870823.
    Abstract:
    Repertoire sequencing of B cells is the high-throughput profiling of B cell receptors (BCR) expressed on the surface of B cells and of immunoglobulins (Ig) expressed by antibody secreting cells. Each BCR/Ig transcript has a unique complementarity-determining region 3 (CDR3) sequence that can be used to identify and track individual B cell lymphocytes over time and throughout different compartments of the human body. B cell differentiation can be further tracked by assessing the point mutations acquired during affinity maturation via somatic hypermutation (SHM). Here we describe a method for high-throughput sequencing of the variable region of Ig heavy-chain transcripts for repertoire analysis of human B cells on the Illumina Miseq platform.
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