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  • Title: The quick-freezing of single intact skeletal muscle fibers at known time intervals following electrical stimulation.
    Author: Nassar R, Wallace NR, Taylor I, Sommer JR.
    Journal: Scan Electron Microsc; 1986; (Pt 1):309-28. PubMed ID: 3488583.
    Abstract:
    Single intact frog skeletal muscle fibers quick-frozen after known time intervals following electrical stimulation are examined electron microscopically in thin sections, after freeze-substitution, in freeze-fracture/etch preparations, and in cryosections prepared for x-ray microprobe analysis. Techniques are described to perform these operations on a single fiber. Notable morphological differences between conventionally fixed and cryopreserved muscle fibers, and between fibers quick-frozen at different post-stimulation intervals are demonstrated.
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