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  • Title: [Yijing Prescription regulates the PI3K/Akt/mTOR signaling pathway and autophagy and inhibits the apoptosis of Leydig cells: An experimental study in vitro].
    Author: Cao Y, Min X, Zhu J, Jia JM.
    Journal: Zhonghua Nan Ke Xue; 2020 Dec; 26(12):1112-1118. PubMed ID: 34898087.
    Abstract:
    OBJECTIVE: To explore the mechanism of Yijing Prescription (YJP) improving sperm quality in oligozoospermia patients. METHODS: Mouse Leydig (TM3) cells were divided into five groups: normal control, acrolein-induced oxidative stress (ACR) model control, and low-, medium- and high-dose YJP. The ACR model was established in the latter four groups with 100 μmol/L acrolein, and the TM3 cells in the latter three groups were treated with the serum containing 5%, 10% and 20% YJP, respectively. After modeling, the viability of the cells and the contents of MDA, T-AOC and SOD were determined by CCK-8, and the relative expressions of PI3K/Akt/mTOR, Beclin-1, Bcl-2 and LC3-II detected by real-time PCR and Western blot. The results obtained were analyzed and compared among different groups. RESULTS: Compared with the normal control cells, the TM3 cells in the ACR model control group showed a dramatically inhibited survival rate (54%), increased content of MDA, decreased levels of T-AOC and SOD (P < 0.01), down-regulated expressions of p-PI3K/p-Akt protein (P < 0.01), and Bcl-2 protein (P < 0.05) and Bcl-2 mRNA (P < 0.01), and up-regulated mRNA and protein expressions of Beclin-1 and LC3II (P < 0.01). After administration of YJP, all the above-mentioned parameters were reversed in different degrees and improved in a dose-dependent manner. CONCLUSIONS: Yijing Prescription acts against oxidative stress, mediates the regulation of the PI3K/Akt/mTOR signaling pathway and autophagy and inhibition of the apoptosis of Leydig cells, and thus improves the sperm quality of oligozoospermia patients.
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