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  • Title: Differential assay of salivary and pancreatic alpha-amylase in serum and urine, with use of monoclonal antibody to human salivary amylase immobilized on bacterial cell wall.
    Author: Hiroishi S, Matsuyama S, Kurooka S, Sunahara N, Inoue S, Ida T.
    Journal: Clin Chem; 1987 Jul; 33(7):1235-6. PubMed ID: 3496178.
    Abstract:
    We previously reported (Clin Chim Acta 1986;159:89) that bacterial cell wall chemically coated with a monoclonal antibody specific to human salivary (S) amylase (EC 3.2.1.1) could be successfully used to separate S and pancreatic (P) amylase in solution. We have now applied this method to serum and urine samples and found that the activities of S and P amylases so measured correlated well with those measured by the isoamylase inhibitor method. The present method is simple and reliable for routine clinical tests.
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