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  • Title: Anti-IgM antibody decreases dissociability of cell bound IgM anti-hapten antibodies as measured with fluid phase hapten.
    Author: Circolo A, Battista P, Borsos T.
    Journal: Mol Immunol; 1987 Jul; 24(7):737-41. PubMed ID: 3498885.
    Abstract:
    We studied the effect of an IgG anti-IgM on the dissociation of cell bound IgM anti-hapten antibody, as measured with fluid phase hapten. Methotrexate (MTX) covalently bound to the red cells surface was used as hapten; rabbit anti-MTX IgM and rabbit anti-allotype IgG reactive with the IgM were used as antibodies. The amount of cell bound antibodies was measured with 125I-labelled protein A. In the absence of the anti-allotype antibody, most of the anti-hapten IgM was prevented from reassociation by fluid phase hapten. In the presence of the anti-allotype IgG most of the anti-hapten IgM was non-dissociable, with about a 1000-fold increase in the apparent binding constant. We found that (1) dissociability of the anti-hapten IgM depended on the density of the cell bound hapten, (2) decrease in dissociability was a function of the anti-allotype antibody concn and (3) even in the presence of excess anti-allotype antibody dissociability was an inverse function of anti-hapten IgM concn.
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