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  • Title: Architecting of an aptasensor for the staphylococcus aureus analysis by modification of the screen-printed carbon electrode with aptamer/Ag-Cs-Gr QDs/NTiO2.
    Author: Ghalkhani M, Sohouli E, Khaloo SS, Vaziri MH.
    Journal: Chemosphere; 2022 Apr; 293():133597. PubMed ID: 35031253.
    Abstract:
    Given the many issues bacterial infections cause to humans and the necessity for their detection, in this work we developed a robust aptasensor for prompt, ultrasensitive, and selective analysis of staphylococcus aureus bacterium (S. aureus). A nanocomposite of Ag nanoparticles, chitosan, graphene quantum dots, and nitrogen-doped TiO2 nanoparticles (Ag-Cs-Gr QDs/NTiO2) was synthesized, and thoroughly characterized by XRD, FT-IR, and FE-SEM spectroscopic methods. The surface of screen-printed carbon electrodes modified with Ag-Cs-Gr QDs/NTiO2 nanocomposite was utilized as a compatible platform for aptamer attachment. The aptasensor accurately determined S. aureus in the dynamic range of 10-5 × 108 CFU/mL with detection limit of 3.3 CFU/mL. The monitoring of the practical performance of aptasensor in human serum samples revealed its superiority over the conventional methods (relative recovery of 96.25-103.33%). The Ag-Cs-Gr QDs/NTiO2-based aptasensor offers facile, biocompatibility, good repeatability, reproducibility (RSD = 3.66%), label free and stabile strategy for sensitive S. aureus analysis free from biomolecules interferences in actual specimens.
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