These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Quantitative immunoprecipitation of the lactose transporter from Escherichia coli confirms stoichiometry of substrate binding.
    Author: Seckler R.
    Journal: Biochem Biophys Res Commun; 1986 Jan 29; 134(2):975-81. PubMed ID: 3511912.
    Abstract:
    A procedure is presented for the immunoprecipitation of the E. coli lactose transporter which may be applicable to other membrane protein antigens. Antibodies to its chemically synthesized C-terminal decapeptide specifically precipitate 6.0 +/- 0.2% of the radioactivity from solubilized [14C]amino-acid-labeled total cell envelopes of the transporter-overproducing strain T206 corresponding to 1.0 +/- 0.1 nmol transporter/mg total membrane protein. Comparison with galactoside binding yields a stoichiometry of 1.1 +/- 0.2 mol galactoside bound/mol transporter.
    [Abstract] [Full Text] [Related] [New Search]