These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Relationship between the Expression of miRNA181a-5p and the Imbalance of Treg/Th17 in Primary Immune Thrombocytopenia].
    Author: Zhao M, Jin LF, Li XF, Zhao Y, Zhu GJ, Shen WZ.
    Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2022 Feb; 30(1):237-243. PubMed ID: 35123634.
    Abstract:
    OBJECTIVE: To investigate the role of relationship between the expression of miRNA181a-5p and imbalance of Treg/Th17 in the pathogenesis of primary immune thrombocytopenia(ITP), which contributes to clarify the mechanism of T cell immune imbalance in ITP patients. METHODS: Peripheral blood was collected from 37 ITP patients, concluding 21 untreated patients and 16 effectively treated patients, and 19 healthy controls; Peripheral blood mononuclear cells (PBMC) were isolated and the expression of miRNA181a-5p and Notch1 was analyzed by RT-PCR. The proportion of Th17 subsets and Treg cells in the peripheral circulation was detected by flow cytometer (FCM). Clinical data of ITP group was collected, including age, platelet count and disease course. RESULTS: The expression of miR-181a-5p was significantly decreased in ITP group than that of healthy control group (P＜0.01). After effective treatment, the expression of miR-181a-5p was significantly higher than that of ITP group (P＜0.05), but still significantly lower than that of healthy control group (P＜0.01); The expression of Notch1 was significantly increased in ITP group and effectively treated group than that of healthy control group (P＜0.01). There was no significant difference in proportion of Treg cells in ITP group, effectively treated group and healthy control group (P＞0.05). The proportion of Th17 subsets in ITP group was significantly increased than that of healthy control group (P＜0.05), while the ratio of Treg/Th17 was significantly decreased (P＜0.05). There was a positive correlation between the expression of miR-181a-5p and ratio of Treg/Th17 in ITP group (r=0.555). CONCLUSION: The expression of miR-181a-5p is significantly decreased in ITP patients, which is closely related to the imbalance of Treg/Th17 cells. After effective treatment, the expression of miR-181a-5p can be significantly corrected, but still failed to reach the level of healthy people. While the expression of Notch1 is significantly increased in ITP patients, and could not reach the level of healthy people after effective treatment. 题目: 原发免疫性血小板减少症miRNA181a-5p表达与Treg/Th17失衡关系的研究. 目的: 研究miRNA181a-5p表达与Treg/Th17失衡关系在原发免疫性血小板减少症（ITP）发病中的作用，有助于阐明ITP患者T细胞免疫失衡的机制. 方法: 收集37例ITP患者（包括21例未治疗患者和16例治疗有效患者）及19例健康对照者的外周血；分离外周血单个核细胞，qRT-PCR分析miRNA181a-5p和Notch1的表达情况；流式细胞术检测ITP患者和健康对照者外周血Th17亚群和Treg细胞比例；收集ITP组的临床数据，包括患者年龄、血小板计数及病程. 结果: ITP组miR-181a-5p表达明显低于对照组（P＜0.01），而经治疗有效后其表达水平明显升高（P＜0.05），但仍明显低于对照组（P＜0.01）；ITP组和治疗有效组Notch1表达明显高于对照组（P＜0.01）。3组之间两两比较，Treg细胞比例均未见明显差异（P＞0.05）；ITP组Th17亚群比例明显高于对照组（P＜0.05），而Treg/Th17比值明显降低（P＜0.05）；ITP组miR-181a-5p表达和Treg/Th17比值呈正相关关系（r=0.555）. 结论: ITP患者存在miR-181a-5p表达明显下降，且与Treg/Th17细胞失衡密切相关，经治疗有效后miR-181a-5p可以得到显著纠正，但仍无法达到健康人水平；而Notch1表达明显升高，经治疗有效后Notch1表达也无法达到健康人水平.
    [Abstract] [Full Text] [Related] [New Search]