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  • Title: Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A.
    Author: Notermans S, Dufrenne J, Schothorst M.
    Journal: Jpn J Med Sci Biol; 1978 Feb; 31(1):81-5. PubMed ID: 351243.
    Abstract:
    The enzyme linked immunosorbent assay using the so-called "double-sandwich technique" has been applied to determine botulinum toxin type A. By this assay, 50-100 mouse ip LD50 of toxin type A can be detected. No cross-reaction occurs with botulinum toxins of other types tested. In all probability this is due to the high specificity of the antiserum prepared against the toxic component of type A toxin.
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