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  • Title: A protocol for investigating lipidomic dysregulation and discovering lipid biomarkers from human serums.
    Author: Chen M, Hao Y, Chen S.
    Journal: STAR Protoc; 2022 Mar 18; 3(1):101125. PubMed ID: 35141563.
    Abstract:
    Lipids play important roles in various human diseases. Disease-associated lipid dysregulation and biomarkers could provide molecular clues for diagnosis, pathogenesis, and therapy. This protocol provides a step-by-step workflow to investigate lipid dysregulation and discover biomarkers in human serum samples by liquid chromatography-mass spectrometry (LC-MS)-based lipidomics and machine learning analysis. The workflow includes project design, serum collection, sample preparation, data acquisition, data processing, and machine learning analysis. For complete details on the use and execution of this profile, please refer to Hao et al. (2021).
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