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  • Title: [Determining the methotrexate concentration in serum with the fluorescence polarization immunoassay (FPIA)--a comparison with the enzyme immunoassay (EMIT)].
    Author: Liappis N.
    Journal: Klin Padiatr; 1986; 198(1):33-6. PubMed ID: 3515034.
    Abstract:
    The determination of methotrexate in serum was performed by the fluorescence polarization immunoassay (FPIA) and the enzyme immunoassay (EMIT). Both methods showed a good precision and accuracy. The investigation was carried out in 138 sera with a methotrexate concentration up to 1,400 mumol/l. The comparison of the two methods in 100 sera with a methotrexate concentration up to 5 mumol/l showed a good correlation (correlation coefficient r = 0.9798). Close correlation (correlation coefficient r = 0.9845) was also found in 38 sera with a methotrexate concentration between 5 and 1,400 mumol/l. Discussion of the FPIA and EMIT methods.
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