These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: BODIPY-dipicolylamine complexes of platinum(II): X-ray structure, cellular imaging and organelle-specific near-IR light type-II PDT.
    Author: Bera A, Gautam S, Raza MK, Pal AK, Kondaiah P, Chakravarty AR.
    Journal: Dalton Trans; 2022 Mar 08; 51(10):3925-3936. PubMed ID: 35170587.
    Abstract:
    Dipicolylamine (dpa) based platinum(II) complexes [Pt(L1-3)Cl]Cl (1-3), where L2 and L3 are green and red light BODIPY-tagged dpa ligands and L1 is a benzyl derivative of dpa, were synthesized and characterized and their in vitro cytotoxicity was studied. The perchlorate salt of complex 2 was structurally characterized. It showed a PtN3Cl core with a deformed square-planar geometry. At pH 7.2, complexes 2 and 3 showed strong absorption bands at 500 nm (ε ∼6.8 × 104 dm3 mol-1 cm-1) and 653 nm (ε ∼1.0 × 105 dm3 mol-1 cm-1) in a 1 : 1 (v/v) mixture of dimethyl sulfoxide and Dulbecco's phosphate-buffered saline (DMSO/DPBS), respectively. They displayed respective emission bands at 515 and 677 nm having fluorescence quantum yield values of 0.36 and 0.25. Complex 3 generated singlet oxygen, as evidenced from the 1,3-diphenylisobenzofuran titration experiments and mechanistic DNA photocleavage study. It showed high photocytotoxicity in red light (600-720 nm) with half-maximal inhibitory concentration (IC50) values of 1.73 and 2.67 μM in HeLa and A549 cells. The complexes showed significantly reduced chemo-PDT activity in a non-cancerous HPL1D cell line and in the dark. The 2',7'-dichlorofluorescein diacetate assay revealed reactive oxygen species-mediated type-II photodynamic therapy (PDT) activity. Cellular imaging of A549 cancer cells using complexes 2 and 3 revealed their preferential localization in mitochondria and endoplasmic reticulum. The annexin V-FITC/PI assay confirmed apoptotic cell damage. Cell cycle analysis indicated arrest in the G1 phase upon red light irradiation. Pt-DNA adduct formation was proposed from a DNA binding experiment with green light active complex 2 and 9-ethylguanine as a nucleobase from the mass spectral study.
    [Abstract] [Full Text] [Related] [New Search]