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  • Title: Use of particulate antigen to enhance primary in vitro antibody responses to trinitrophenyl-keyhole limpet hemocyanin.
    Author: Buttke TM, Pourbohloul S, Miller NW.
    Journal: J Immunol Methods; 1986 May 22; 89(2):233-8. PubMed ID: 3517178.
    Abstract:
    Methods are described for the efficient induction of primary anti-hapten antibody responses to trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH) in vitro. Mouse leukocytes stimulated in vitro with particulate (bentonite-adsorbed) TNP-KLH demonstrated 10-fold more IgM-secreting, plaque-forming cells (PFC) as measured by a hemolytic plaque assay than did leukocytes stimulated with soluble TNP-KLH. The T dependency of the enhanced antibody response was confirmed using highly enriched B and T lymphocyte populations. These methods should greatly facilitate studies regarding primary in vitro antibody responsiveness to T-dependent antigens.
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