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  • Title: Human articular cartilage secretes characteristic metal dependent proteinases upon stimulation by mononuclear cell factor.
    Author: Campbell IK, Golds EE, Mort JS, Roughley PJ.
    Journal: J Rheumatol; 1986 Feb; 13(1):20-7. PubMed ID: 3517323.
    Abstract:
    Human articular cartilage stimulated during organ culture by the addition of human blood mononuclear cell factor concomitantly released latent proteinases and proteoglycan. Gel chromatography at pH 7.4 separated 2 distinct latent enzymes: a general proteinase which could degrade proteoglycan, casein and gelatin, and a collagenolytic activity of smaller molecular size. The general proteinase was calcium dependent and could be activated by either trypsin or 4-aminophenylmercuric acetate; the former procedure invariably generating greatest activity. The enzyme exhibited a broad bimodal pH profile against proteoglycan, with optimum activity at pH 5.5.
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