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  • Title: [Preparation of iduronate sulfatase from the human placenta].
    Author: Di Natale P, Murino P, Perfumo A.
    Journal: Boll Soc Ital Biol Sper; 1978 Sep 15; 54(17):1587-91. PubMed ID: 35184.
    Abstract:
    The preparation of the enzyme iduronate sulfatase from human placenta has been undertaken. The substrate O-(alpha-L-idopyranosyluronic acid 2-sulfate) (1 leads to 4)-2,5-anhydro-D-[3H]mannitol 6-sulfate was used to measure the enzymatic activity. The enzyme shows a pH optimum of 4.0 in 0.1 M sodium formiate or acetate buffer. Chromatography on DE-52 gives a 5.4 fold purification. The enzyme is inhibited by NaCl or KCl: in 20 mM salt the reaction rate was only 63% and 34% respectively. Inhibition by salt can be removed by extensive dialysis after the chromatographic step.
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